7NDY
Di-phosphorylated Barrier-to-Autointegration Factor (BAF) in complex with LEM domain of Emerin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2020-11-25 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.87313 |
Spacegroup name | P 1 |
Unit cell lengths | 33.050, 40.628, 43.224 |
Unit cell angles | 69.51, 69.68, 85.03 |
Refinement procedure
Resolution | 38.030 - 1.440 |
R-factor | 0.1829 |
Rwork | 0.181 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ghd |
RMSD bond length | 0.008 |
RMSD bond angle | 0.870 |
Data reduction software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.225 | 1.411 |
High resolution limit [Å] | 1.242 | 1.242 |
Rmerge | 0.802 | |
Rmeas | 0.965 | |
Rpim | 0.527 | |
Number of reflections | 32449 | 1622 |
<I/σ(I)> | 4.5 | 1.3 |
Completeness [%] | 84.8 | |
Redundancy | 15 | |
CC(1/2) | 0.989 | 0.326 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | Hepes Sodium salt 0.1 M pH 7.5 PEG 600 25% W/V |