7N0A

Structure of Human Leukaemia Inhibitory Factor with Fab MSC1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332013463416092
Spacegroup name	P 21 21 2
Unit cell lengths	60.572, 109.380, 115.691
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.740 - 3.100
R-factor	0.2345
Rwork	0.232
R-free	0.28400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pvh
RMSD bond length	0.002
RMSD bond angle	0.534
Data reduction software	XPREP
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.740	3.210
High resolution limit [Å]	3.100	3.100
Number of reflections	14528	1427
<I/σ(I)>	5.1
Completeness [%]	98.4
Redundancy	12
CC(1/2)	0.975	0.597

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	277.15	19% (v/v) isopropanol, 19% (w/v) PEG 4000, 5% (v/v) glycerol, 0.095 M sodium citrate, pH 5.6