7MXI

IgE-Fc in complex with DARPins E2_79 and E3_53

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	51.908, 137.793, 88.250
Unit cell angles	90.00, 105.68, 90.00

Refinement procedure

Resolution	38.740 - 2.800
R-factor	0.2152
Rwork	0.213
R-free	0.25630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gt7 4grg 4ycw
RMSD bond length	0.006
RMSD bond angle	0.998
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.740	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.195	0.824
Number of reflections	29053	2901
<I/σ(I)>	5.07	0.66
Completeness [%]	98.7	98.37
Redundancy	3.7
CC(1/2)	0.983	0.577

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	291	E3_53, E2_79, and IgE-Fc3-4 were mixed in equimolar ratios with E3_53 and E2_79, and intact complexes were purified by SEC in 20 mM Tris-HCl, pH 8.0, 50mM NaCl and concentrated to 7mg/ml and mixed 1:1 with acetate buffer pH 4.6, 43% ethylene glycol w/v in hanging drops