7MSE

High-resolution crystal structure of hMIF2 with tartrate at the active site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-30
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.51418
Spacegroup name	P 3
Unit cell lengths	83.396, 83.396, 40.429
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.700 - 1.270
R-factor	0.1388
Rwork	0.137
R-free	0.17290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kan
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.18)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.290
High resolution limit [Å]	1.270	3.450	1.270
Rmerge	0.077	0.033	0.605
Rmeas	0.080	0.034	0.701
Rpim	0.023	0.008	0.341
Total number of observations	933698
Number of reflections	82635	4158	3642
<I/σ(I)>	8.7
Completeness [%]	99.3	100	86.8
Redundancy	11.3	19.1	3.4
CC(1/2)		1.000	0.638

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	28.6% w/v PEG 3350, 0.31M sodium tartrate at pH 5.6