7MRV

F100A mutant structure of MIF2 (D-DT)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-08
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.514
Spacegroup name	H 3
Unit cell lengths	83.161, 83.161, 37.781
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.580 - 1.570
R-factor	0.1693
Rwork	0.167
R-free	0.21110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kan
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.18)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.600
High resolution limit [Å]	1.570	4.260	1.570
Rmerge	0.030	0.032	0.129
Rmeas	0.033	0.034	0.152
Rpim	0.012	0.011	0.079
Total number of observations	70639
Number of reflections	13566	683	688
<I/σ(I)>	31.4
Completeness [%]	99.9	100	98.4
Redundancy	5.2	8.9	3.3
CC(1/2)		0.999	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.2M Ammonium sulfate, 25% PEG3350, 0.1M Tris