7MNW
Crystal Structure of Nup358/RanBP2 Ran-binding domain 1 in complex with Ran-GPPNHP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-18 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 1.00000 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 137.370, 137.834, 172.934 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.960 - 2.400 |
R-factor | 0.2016 |
Rwork | 0.200 |
R-free | 0.24200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rrp |
Data reduction software | XDS |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.960 | 28.960 | 2.490 |
High resolution limit [Å] | 2.400 | 5.160 | 2.400 |
Rmeas | 0.306 | 0.122 | 2.847 |
Rpim | 0.082 | 0.034 | 0.750 |
Total number of observations | 880269 | 89717 | 87689 |
Number of reflections | 64146 | 6687 | 6219 |
<I/σ(I)> | 6.7 | 13.9 | 1.3 |
Completeness [%] | 99.8 | 100 | 98.2 |
Redundancy | 13.7 | 13.4 | 14.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 20% w/v PEG3350, 0.2 M potassium sodium tartrate tetrahydrate |