7MDL
High-resolution crystal structure of human SepSecS-tRNASec complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2013-08-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 167.058, 167.058, 239.846 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.600 - 2.320 |
| R-factor | 0.1763 |
| Rwork | 0.176 |
| R-free | 0.20250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hl2 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.619 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (3951) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.360 |
| High resolution limit [Å] | 2.320 | 6.290 | 2.320 |
| Rmerge | 0.133 | 0.055 | 1.501 |
| Rmeas | 0.144 | 0.059 | 1.704 |
| Rpim | 0.053 | 0.021 | 0.789 |
| Total number of observations | 1176629 | ||
| Number of reflections | 165776 | 8530 | 8185 |
| <I/σ(I)> | 7.9 | ||
| Completeness [%] | 99.9 | 99.6 | 99.6 |
| Redundancy | 7.1 | 8 | 4.5 |
| CC(1/2) | 0.998 | 0.403 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 285 | 0.24 M lithium citrate, 10% PEG 3350 0.1 M, sodium cacodylate trihydrate, 2.0 mg/mL SepSecS mixed with 0.5 mg/mL tRNASec |
