7M8H

Structure of Memo1 C244S metal binding site mutant at 1.75A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-03-12
Detector	RAYONIX MX-300
Wavelength(s)	0.968
Spacegroup name	P 21 21 2
Unit cell lengths	140.000, 89.520, 97.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.630 - 1.750
R-factor	0.1556
Rwork	0.154
R-free	0.18720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7kq8
Data reduction software	AutoProcess
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.630	42.630	1.860
High resolution limit [Å]	1.750	5.000	1.750
Rmerge	0.088	0.026	1.052
Rmeas	0.091	0.027	1.091
Number of reflections	238611	5654	20426
<I/σ(I)>	27.83	75.54	3.2
Completeness [%]	99.9	99.7	99.8
Redundancy	14.819	14.169	14.272
CC(1/2)	1.000	1.000	0.893

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	292	0.1 M MES pH 5.5-7.0 22 % PEG3350 under anaerobic conditions