7LTT
SAMHD1(113-626) H206R D207N R366C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.926, 140.105, 97.193 |
| Unit cell angles | 90.00, 114.18, 90.00 |
Refinement procedure
| Resolution | 39.380 - 1.900 |
| R-factor | 0.1792 |
| Rwork | 0.177 |
| R-free | 0.21160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bzb |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.698 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.071 | 1.311 |
| Number of reflections | 153730 | 7144 |
| <I/σ(I)> | 17.3 | |
| Completeness [%] | 98.9 | 98.8 |
| Redundancy | 3.3 | 3.3 |
| CC(1/2) | 0.996 | 0.297 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8.2 | 293 | PEG 1500, dGTP, SPG buffer, sodium chloride, magnesium chloride, TCEP |
