7LS0

Structure of the Human ALK GRD bound to AUG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	P 1 21 1
Unit cell lengths	66.262, 177.754, 94.855
Unit cell angles	90.00, 104.80, 90.00

Refinement procedure

Resolution	49.770 - 3.050
R-factor	0.2403
Rwork	0.238
R-free	0.28820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7lrz
RMSD bond length	0.005
RMSD bond angle	0.766
Data reduction software	XDS (20200417)
Data scaling software	SCALA (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (dev_3915)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.770	3.120
High resolution limit [Å]	3.050	3.050
Rmerge	0.166	0.783
Number of reflections	40292	2737
<I/σ(I)>	3.6	1.3
Completeness [%]	99.6	87.1
Redundancy	3.3	3.3
CC(1/2)	0.999	0.400

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	289.15	Citrate pH 5.5, 20% PEG 3000