7LMM

Crystal structure of bovine DNMT1 BAH1 domain in complex with H4K20me2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-09
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	P 2 21 21
Unit cell lengths	70.696, 80.281, 130.424
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.906 - 2.798
R-factor	0.2125
Rwork	0.207
R-free	0.26680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wxx
RMSD bond length	0.008
RMSD bond angle	0.962
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.910	2.900
High resolution limit [Å]	2.790	2.800
Number of reflections	18810	1823
<I/σ(I)>	20.8
Completeness [%]	99.5
Redundancy	12.9
CC(1/2)	0.998	0.866

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	0.1 M MES (pH 6.5) ,13% PEG1500, 20 mM DTT and 200 mM L-proline