7LET

Structure of importin a2 bound to the p50- and p65-NLSs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	77.944, 91.273, 97.242
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.400
R-factor	0.2003
Rwork	0.198
R-free	0.24150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1y2a
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rpim	0.080	0.708
Number of reflections	24702	1570
<I/σ(I)>	13.2
Completeness [%]	89.2	57.8
Redundancy	3.1	2.2
CC(1/2)	0.988	0.409

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298	0.6 Na Citrate, 100 mM HEPES pH 6