7LBW

Crystal structure of TFAM (mitochondrial transcription factor A) bridging two non-sequence specific DNA substrates

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-27
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1
Spacegroup name	P 21 21 2
Unit cell lengths	115.000, 124.810, 55.179
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.810 - 2.840
R-factor	0.2004
Rwork	0.199
R-free	0.23210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tq6
RMSD bond length	0.013
RMSD bond angle	1.421
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.810	2.850
High resolution limit [Å]	2.840	2.840
Rmerge	0.061	0.636
Number of reflections	19494	179
<I/σ(I)>	33.6
Completeness [%]	100.0
Redundancy	12.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.1 M Bis-Tris (pH 6.5) and 24% PEG 2000 MME