7LBC
Structure of human GGT1 in complex with Lnt2-65 compound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X17B1 |
| Synchrotron site | NSLS |
| Beamline | X17B1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-23 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.92009 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 105.216, 122.681, 102.507 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 79.870 - 2.280 |
| R-factor | 0.2522 |
| Rwork | 0.249 |
| R-free | 0.30570 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4z9o |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.250 |
| Data reduction software | XDS |
| Data scaling software | pointless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.870 | 2.340 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.149 | 0.780 |
| Number of reflections | 30603 | 4259 |
| <I/σ(I)> | 10.2 | 2.5 |
| Completeness [%] | 99.6 | 95.2 |
| Redundancy | 10.1 | 9.6 |
| CC(1/2) | 0.997 | 0.911 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 22% PEG 3350, 0.1M ammonium chloride, 0.1M sodium cacodylate |
