7L05

Complex of novel maytansinoid M24 bound to T2R-TTL (two tubulin alpha/beta heterodimers, RB3 stathmin-like domain, and tubulin tyrosine ligase)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-20
Detector	DECTRIS PILATUS 300K
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	105.088, 158.036, 181.014
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.620 - 2.210
R-factor	0.1873
Rwork	0.186
R-free	0.21670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ihj
RMSD bond length	0.009
RMSD bond angle	1.543
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.077	0.932
Rmeas	0.081	0.984
Rpim	0.023	0.311
Number of reflections	150790	7363
<I/σ(I)>	33	2.2
Completeness [%]	99.4	98.2
Redundancy	12.2	9.4
CC(1/2)		0.748

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	PEG 3350, MES, CaCl2, MgCl2, glycerol