7KQN
Ternary complex of TERT (telomerase reverse transcriptase) with RNA template, DNA primer, an incoming dGTP and a downstream hybrid duplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-07-27 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.860, 164.040, 95.360 |
| Unit cell angles | 90.00, 111.91, 90.00 |
Refinement procedure
| Resolution | 29.650 - 2.020 |
| R-factor | 0.1677 |
| Rwork | 0.168 |
| R-free | 0.18780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kyl |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.228 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.130 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Number of reflections | 131028 | 9762 |
| <I/σ(I)> | 10.55 | 1.07 |
| Completeness [%] | 97.9 | |
| Redundancy | 3.7 | |
| CC(1/2) | 0.998 | 0.433 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.15 M Potassium Chloride, 0.05 M MES (pH 6.0), 2% PEG 8000 |
