7KQK
anti-pTau C21-ABS Fab in complex with pTau peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-07-15 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.370, 76.580, 119.470 |
| Unit cell angles | 90.00, 90.36, 90.00 |
Refinement procedure
| Resolution | 64.470 - 2.600 |
| R-factor | 0.212 |
| Rwork | 0.211 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4glr |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.170 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.600 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.079 | 0.410 |
| Number of reflections | 29183 | 4234 |
| <I/σ(I)> | 9.5 | |
| Completeness [%] | 99.7 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 300 | 20 % PEG 3350 (w/v) and 150 mM DL-malic acid. |
