7KJU

Cgi121-tRNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-21
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97964
Spacegroup name	C 2 2 2
Unit cell lengths	100.493, 168.527, 68.968
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.034 - 3.102
R-factor	0.2492
Rwork	0.248
R-free	0.26650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3l0u
Data reduction software	DIALS (1.14.5)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.16)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.140	49.140	3.190
High resolution limit [Å]	3.100	13.910	3.110
Rmerge	0.081	0.036	0.931
Rmeas	0.085	0.038	0.989
Rpim	0.025	0.012	0.330
Total number of observations		1211	7016
Number of reflections	10895	127	792
<I/σ(I)>	13.3	36.8	2.1
Completeness [%]	99.9	87.2	100
Redundancy	11.8	9.5	8.9
CC(1/2)	0.999	0.999	0.956

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	100 mM MES, pH 6.0, 0.2 M zinc acetate, 10% PEG8000