7KJU
Cgi121-tRNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-21 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97964 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 100.493, 168.527, 68.968 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.030 - 3.100 |
| R-factor | 0.2283 |
| Rwork | 0.227 |
| R-free | 0.24590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3l0u |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.452 |
| Data reduction software | DIALS (1.14.5) |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX ((1.21.1_5286: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.140 | 49.140 | 3.190 |
| High resolution limit [Å] | 3.100 | 13.910 | 3.110 |
| Rmerge | 0.081 | 0.036 | 0.931 |
| Rmeas | 0.085 | 0.038 | 0.989 |
| Rpim | 0.025 | 0.012 | 0.330 |
| Total number of observations | 1211 | 7016 | |
| Number of reflections | 10895 | 127 | 792 |
| <I/σ(I)> | 13.3 | 36.8 | 2.1 |
| Completeness [%] | 99.9 | 87.2 | 100 |
| Redundancy | 11.8 | 9.5 | 8.9 |
| CC(1/2) | 0.999 | 0.999 | 0.956 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 100 mM MES, pH 6.0, 0.2 M zinc acetate, 10% PEG8000 |
