7KJU

Cgi121-tRNA complex

Summary for 7KJU

• Entry DOI: 10.2210/pdb7kju/pdb
• Related: 7KJT
• Descriptor: RNA (75-MER) (1 entity in total)
• Functional Keywords: keops, trna, cgi121, rna modifying enzyme, n6-threonylcarbamoyl adenosine, rna
• Biological source: Methanocaldococcus jannaschii
• Total number of polymer chains: 1
• Total formula weight: 24758.67

Authors

Ceccarelli, D.F.,Beenstock, J.,Wan, L.C.K.,Sicheri, F. (deposition date: 2020-10-26, release date: 2020-12-02, Last modification date: 2025-06-25)

Primary citation

Beenstock, J.,Ona, S.M.,Porat, J.,Orlicky, S.,Wan, L.C.K.,Ceccarelli, D.F.,Maisonneuve, P.,Szilard, R.K.,Yin, Z.,Setiaputra, D.,Mao, D.Y.L.,Khan, M.,Raval, S.,Schriemer, D.C.,Bayfield, M.A.,Durocher, D.,Sicheri, F.
A substrate binding model for the KEOPS tRNA modifying complex.
Nat Commun, 11:6233-6233, 2020

PubMed Abstract: The KEOPS complex, which is conserved across archaea and eukaryotes, is composed of four core subunits; Pcc1, Kae1, Bud32 and Cgi121. KEOPS is crucial for the fitness of all organisms examined. In humans, pathogenic mutations in KEOPS genes lead to Galloway-Mowat syndrome, an autosomal-recessive disease causing childhood lethality. Kae1 catalyzes the universal and essential tRNA modification N-threonylcarbamoyl adenosine, but the precise roles of all other KEOPS subunits remain an enigma. Here we show using structure-guided studies that Cgi121 recruits tRNA to KEOPS by binding to its 3' CCA tail. A composite model of KEOPS bound to tRNA reveals that all KEOPS subunits form an extended tRNA-binding surface that we have validated in vitro and in vivo to mediate the interaction with the tRNA substrate and its modification. These findings provide a framework for understanding the inner workings of KEOPS and delineate why all KEOPS subunits are essential.

PubMed: 33277478
DOI: 10.1038/s41467-020-19990-5

Experimental method

X-RAY DIFFRACTION (3.1 Å)