7KJC
Crystal structure of the EphA2 S901E mutant intracellular KD-SAM domains
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 287 |
Detector technology | IMAGE PLATE |
Collection date | 2014-12-19 |
Detector | RIGAKU RAXIS HTC |
Wavelength(s) | 1.5418 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 121.639, 54.587, 135.538 |
Unit cell angles | 90.00, 95.22, 90.00 |
Refinement procedure
Resolution | 29.250 - 2.300 |
R-factor | 0.1903 |
Rwork | 0.188 |
R-free | 0.22570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4pdo 3kka |
RMSD bond length | 0.002 |
RMSD bond angle | 0.520 |
Data reduction software | XDS (VERSION January 10, 2014) |
Data scaling software | Aimless (version 0.5.32) |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.250 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.090 | 0.656 |
Rmeas | 0.125 | 0.905 |
Rpim | 0.086 | 0.621 |
Number of reflections | 39708 | 3887 |
<I/σ(I)> | 12 | 1.8 |
Completeness [%] | 99.8 | |
Redundancy | 3.7 | |
CC(1/2) | 0.995 | 0.643 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 287 | 0.2 M MgCl2, 0.1 M Bis-Tris pH5.5, 25% PEG 3350 |