7KG9
Dihydrodipicolinate synthase (DHDPS) from C.jejuni, H56W mutant with pyruvate bound in the active site and L-lysine bound at the allosteric site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-10-11 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 84.830, 225.170, 200.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.042 - 2.060 |
| R-factor | 0.2777 |
| Rwork | 0.275 |
| R-free | 0.33080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ly8 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.647 |
| Data reduction software | HKL-3000 |
| Data scaling software | XSCALE |
| Phasing software | AMPLE |
| Refinement software | PHENIX (dev_2398) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.042 | 2.134 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.852 | |
| Number of reflections | 109047 | 10111 |
| <I/σ(I)> | 3.47 | |
| Completeness [%] | 92.0 | 85.99 |
| Redundancy | 15 | |
| CC(1/2) | 0.940 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.3 | 288.15 | 0.2 M Magnesium acetate, 14 % PEG8000 (pH 7.3) |
