7KBT
Factor VIII in complex with the anti-C2 domain antibody, G99
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2019-08-29 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 132.243, 132.243, 380.029 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.480 - 4.150 |
R-factor | 0.302 |
Rwork | 0.300 |
R-free | 0.33840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6mf0 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.074 |
Data reduction software | Adxv |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.16_3549: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.480 | 4.298 |
High resolution limit [Å] | 4.150 | 4.150 |
Rmerge | 0.064 | 1.359 |
Rmeas | 0.090 | 1.922 |
Rpim | 0.064 | 1.359 |
Number of reflections | 26284 | 2546 |
<I/σ(I)> | 6.31 | 0.48 |
Completeness [%] | 99.1 | 98.31 |
Redundancy | 2 | 2 |
CC(1/2) | 0.998 | 0.373 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 8 - 18% PEG 1500, PEG 6K, or PEG 10K and 50mM malic acid, pH 7. |