7K4H
Human Arginase 1 in complex with compound 04.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00003 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.140, 286.090, 66.952 |
Unit cell angles | 90.00, 90.12, 90.00 |
Refinement procedure
Resolution | 48.880 - 1.650 |
R-factor | 0.212 |
Rwork | 0.210 |
R-free | 0.23500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6v7e |
RMSD bond length | 0.013 |
RMSD bond angle | 1.450 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | BUSTER |
Refinement software | BUSTER (2.11.7) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 71.523 | 1.673 |
High resolution limit [Å] | 1.645 | 1.645 |
Number of reflections | 218547 | 11371 |
<I/σ(I)> | 8.6 | |
Completeness [%] | 90.9 | |
Redundancy | 3.5 | |
CC(1/2) | 0.970 | 0.731 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 10% MMT (pH 7.0) 0.1 M ammonium formate, 16-22% PEG 8000 |