7K02

The crystal structure of a BAK dimer activated by detergent

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	C 1 2 1
Unit cell lengths	157.320, 115.610, 44.530
Unit cell angles	90.00, 90.06, 90.00

Refinement procedure

Resolution	39.330 - 3.400
R-factor	0.2983
Rwork	0.296
R-free	0.34270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6uxm
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.330	3.522
High resolution limit [Å]	3.400	3.400
Rmeas	0.057	1.222
Number of reflections	11007	1083
<I/σ(I)>	13.43	1.32
Completeness [%]	99.6
Redundancy	3.9
CC(1/2)	0.999	0.484

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	291	50% (v/v) methane pentane diol (MPD) and 0.1 M imidazole pH 7.0