7JOO
Crystal structure of ICOS in complex with antibody STIM003 and anti-kappa VHH domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979341 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 171.855, 49.031, 91.817 |
| Unit cell angles | 90.00, 101.90, 90.00 |
Refinement procedure
| Resolution | 28.480 - 2.400 |
| R-factor | 0.244 |
| Rwork | 0.241 |
| R-free | 0.29660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i8l internal database |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.608 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.480 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.109 | 0.608 |
| Rpim | 0.045 | 0.258 |
| Number of reflections | 30150 | 3223 |
| <I/σ(I)> | 13.3 | 2.1 |
| Completeness [%] | 99.2 | 94 |
| Redundancy | 6.6 | 6 |
| CC(1/2) | 0.997 | 0.732 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2 M di-sodium hydrogen phosphate and 20% (w/v) polyethylene glycol 3350 |
