7JL8

Human PrimPol extending from the correct primer base C opposite the 8-oxoguanine lesion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.03324
Spacegroup name	P 1
Unit cell lengths	50.689, 65.330, 72.001
Unit cell angles	68.47, 85.67, 86.92

Refinement procedure

Resolution	28.160 - 2.100
R-factor	0.1883
Rwork	0.187
R-free	0.21330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5l2x
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.162	2.160
High resolution limit [Å]	2.099	2.100
Number of reflections	48622	3825
<I/σ(I)>	11.6
Completeness [%]	97.1	93.6
Redundancy	3.4
CC(1/2)	0.998	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	225-250 mM CaCl2, 16-19% PEG 3350