7JK1
Human PrimPol inserting correct dCTP opposite the 8-oxoguanine lesion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-08-16 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979180 |
Spacegroup name | P 1 |
Unit cell lengths | 52.558, 65.732, 75.179 |
Unit cell angles | 69.66, 82.83, 88.54 |
Refinement procedure
Resolution | 39.877 - 2.620 |
R-factor | 0.2416 |
Rwork | 0.239 |
R-free | 0.28580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5l2x |
RMSD bond length | 0.011 |
RMSD bond angle | 0.681 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.950 | 2.740 |
High resolution limit [Å] | 2.620 | 2.620 |
Rmerge | 0.112 | 1.189 |
Number of reflections | 27758 | 3360 |
<I/σ(I)> | 6.1 | |
Completeness [%] | 98.7 | 98 |
Redundancy | 2.1 | |
CC(1/2) | 0.988 | 0.375 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 225-250 mM CaCl2 16-19% PEG 3350 |