7F7W
JAK2-JH2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.988 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.980, 57.770, 86.240 |
| Unit cell angles | 90.00, 109.32, 90.00 |
Refinement procedure
| Resolution | 57.546 - 1.830 |
| R-factor | 0.2076 |
| Rwork | 0.207 |
| R-free | 0.23830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ut5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.000 |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.000 | 1.980 |
| High resolution limit [Å] | 1.830 | 1.950 |
| Number of reflections | 48992 | 4195 |
| <I/σ(I)> | 14.5 | |
| Completeness [%] | 94.4 | |
| Redundancy | 6.4 | |
| CC(1/2) | 0.999 | 0.968 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1M Tris, pH 8.0 0.2M Sodium acetate, 12-20% PEG 4000 |
