7EZC
Adenosine A2a receptor mutant-I92N
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2020-07-12 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 71.230, 175.730, 112.710 |
Unit cell angles | 90.00, 91.22, 90.00 |
Refinement procedure
Resolution | 47.430 - 3.800 |
R-factor | 0.289 |
Rwork | 0.288 |
R-free | 0.31300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3qak |
RMSD bond length | 0.003 |
RMSD bond angle | 0.635 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.429 | 3.940 |
High resolution limit [Å] | 3.800 | 3.800 |
Number of reflections | 13682 | |
<I/σ(I)> | 10.46 | 1.52 |
Completeness [%] | 99.9 | |
Redundancy | 43.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 8.2 | 291 | 100 mM Tris pH 8.2, 30% PEG400 and 0.4 M (NH4)2SO4. |