7EX8

Crystal structure of Ebinur Lake virus cap snatching endonuclease in complex with inhibitor 27

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	40.748, 70.692, 71.063
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.780 - 1.970
R-factor	0.1967
Rwork	0.195
R-free	0.22690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xi5
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4080)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.040
High resolution limit [Å]	1.970	4.240	1.970
Rmerge	0.095	0.072	0.408
Rmeas	0.107	0.082	0.476
Rpim	0.048	0.038	0.239
Total number of observations	70936
Number of reflections	14633	1620	1232
<I/σ(I)>	8.9
Completeness [%]	96.8	98.1	82.7
Redundancy	4.8	4.6	3.4
CC(1/2)		0.993	0.892

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.7	289	PEG 3350