7EQ2

Crystal structure of GDP-bound Rab1a-T75D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.977760
Spacegroup name	P 1
Unit cell lengths	39.489, 50.598, 52.626
Unit cell angles	76.94, 84.62, 86.27

Refinement procedure

Resolution	25.116 - 1.551
R-factor	0.161457428126
Rwork	0.160
R-free	0.18519
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fol
RMSD bond length	0.006
RMSD bond angle	0.902
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.120	1.610
High resolution limit [Å]	1.551	1.551
Number of reflections	54416	5434
<I/σ(I)>	42.71
Completeness [%]	95.2
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		291.15	40mM Lithium chloride, 20mM Magnesium chloride hexahydrate, 80mM NaCl, 2mM Hexammine cobalt(III) chloride, 40mM Sodium cacodylate trihydrate pH 5.5, 30% v/v (+/-)-2-Methyl-2,4-pentanediol