7ELW

Crystal structure of RNase L in complex with Myricetin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-28
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.5406
Spacegroup name	P 21 21 21
Unit cell lengths	59.608, 111.925, 264.929
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.920 - 3.550
R-factor	0.2425
Rwork	0.240
R-free	0.28040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m11
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.920	3.676
High resolution limit [Å]	3.550	3.550
Number of reflections	22127	2166
<I/σ(I)>	13.03
Completeness [%]	99.3
Redundancy	3.8
CC(1/2)	0.993	0.885

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	18% PEG4000, 100 mM Tris, 150 mM (NH4)2SO4 and 2 mM DTT Buffer, pH7.0