7ECA

Crystal structure of the Keap1 complex with a peptide base on ETGE motif.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	CCD
Collection date	2020-07-17
Detector	OXFORD ONYX CCD
Wavelength(s)	1.54184
Spacegroup name	P 61
Unit cell lengths	102.814, 102.814, 54.812
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.135 - 2.000
R-factor	0.198818141711
Rwork	0.197
R-free	0.23546
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1x2r
RMSD bond length	0.002
RMSD bond angle	0.577
Data reduction software	CrysalisPro (1.171.40.54a)
Data scaling software	CCP4 (7.0.053)
Phasing software	CCP4 (7.0.053)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.135	2.110
High resolution limit [Å]	2.000	2.000
Number of reflections	22476	164132
<I/σ(I)>	1.96
Completeness [%]	99.8
Redundancy	8.4
CC(1/2)	0.996	0.994

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	Lithium Sulfate,Ammonium Sulfate,sodium citrate 5.1