7EA9

Crystal Structure of human lysyl-tRNA synthetase Y145H mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9788
Spacegroup name	P 32
Unit cell lengths	152.025, 152.025, 108.453
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.500
R-factor	0.1657
Rwork	0.163
R-free	0.20990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bju
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.13)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.590
High resolution limit [Å]	2.500	5.380	2.500
Rmerge	0.113	0.060	0.570
Rmeas	0.125	0.066	0.629
Rpim	0.052	0.027	0.264
Number of reflections	97279	9708	9754
<I/σ(I)>	5.3
Completeness [%]	99.7	99.5	100
Redundancy	5.7	5.8	5.6
CC(1/2)		0.996	0.825

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.08 M Magnesium acetate tetrahydrate, 0.1 M sodium citrate (pH 6.0) and 14% (W/V) PEG 5000 MME.