7E9B

Structural basis of HLX10 PD-1 receptor recognition, a promising anti-PD-1 antibody clinical candidate for cancer immunotherapy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9751
Spacegroup name	P 1
Unit cell lengths	38.985, 45.014, 80.890
Unit cell angles	93.60, 102.16, 97.34

Refinement procedure

Resolution	31.410 - 1.780
R-factor	0.1716
Rwork	0.170
R-free	0.20980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zqk
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18_3855)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.414	1.840
High resolution limit [Å]	1.779	1.779
Number of reflections	48174	48174
<I/σ(I)>	28.9
Completeness [%]	94.6
Redundancy	7.1
CC(1/2)	0.989	0.989

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M Tris pH 7.5 containing 23% (w/v) PEG 4000