7E8M
Crystal structure of SARS-CoV-2 antibody P2C-1F11 with mutated RBD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9796 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 195.844, 85.973, 57.872 |
Unit cell angles | 90.00, 99.75, 90.00 |
Refinement procedure
Resolution | 26.170 - 2.090 |
R-factor | 0.1704 |
Rwork | 0.169 |
R-free | 0.19860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7cdi |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.170 | 2.170 |
High resolution limit [Å] | 2.090 | 2.090 |
Number of reflections | 50710 | 3033 |
<I/σ(I)> | 17.2 | |
Completeness [%] | 90.9 | |
Redundancy | 5.3 | |
CC(1/2) | 0.997 | 0.762 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2M Ammonium sulfate, 0.1M Tris pH 8.5, 12%w/v PEG 8000 |