7E7C
Crystal structure of ENL YEATS domain T1 mutant in complex with histone H3 acetylation at K27
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-11-02 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.9779 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 38.635, 118.065, 34.350 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.350 - 1.840 |
R-factor | 0.2189 |
Rwork | 0.215 |
R-free | 0.25130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5j9s |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.870 |
High resolution limit [Å] | 1.840 | 4.990 | 1.840 |
Rmerge | 0.129 | 0.081 | 0.816 |
Rmeas | 0.138 | 0.088 | 0.852 |
Rpim | 0.047 | 0.035 | 0.244 |
Total number of observations | 141761 | ||
Number of reflections | 14147 | 803 | 659 |
<I/σ(I)> | 7.2 | ||
Completeness [%] | 98.8 | 98 | 99.1 |
Redundancy | 10 | 6.4 | 12.1 |
CC(1/2) | 0.992 | 0.876 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 25% PEG 4000, 0.1M MES pH 6.5, 0.2M Potassium iodide |