7DR4
Complex of anti-human IL-2 antibody and human IL-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 11C |
Synchrotron site | PAL/PLS |
Beamline | 11C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-21 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 79.334, 72.261, 210.930 |
Unit cell angles | 90.00, 92.71, 90.00 |
Refinement procedure
Resolution | 29.790 - 2.490 |
R-factor | 0.1964 |
Rwork | 0.195 |
R-free | 0.25770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5lqb |
RMSD bond length | 0.008 |
RMSD bond angle | 1.014 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.17.1_3660) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.250 | 2.500 |
High resolution limit [Å] | 2.490 | 2.500 |
Rmeas | 0.179 | |
Rpim | 0.083 | |
Number of reflections | 80414 | 5025 |
<I/σ(I)> | 1.34 | |
Completeness [%] | 96.3 | |
Redundancy | 4.3 | |
CC(1/2) | 0.980 | 0.442 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M NH4NO3, pH4.8, 25% PEG3350 |