7DHG
Crystal structure of SARS-CoV-2 Orf9b complex with human TOM70
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-10 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.655, 79.438, 124.027 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.010 - 2.200 |
R-factor | 0.2263 |
Rwork | 0.224 |
R-free | 0.26680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7kdt |
RMSD bond length | 0.002 |
RMSD bond angle | 0.444 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.890 | 2.330 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.099 | 1.350 |
Number of reflections | 51880 | 7594 |
<I/σ(I)> | 10.33 | 0.71 |
Completeness [%] | 98.1 | 88.9 |
Redundancy | 5.78 | 2.99 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 296 | 0.1m Bis-tris ph5.5,21%PEG3350 |