7CRU

hnRNPK NLS in complex with Importin alpha 1 (KPNA2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-10-08
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	89.792, 83.317, 101.372
Unit cell angles	90.00, 98.24, 90.00

Refinement procedure

Resolution	46.680 - 2.800
R-factor	0.215
Rwork	0.214
R-free	0.24050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wv6
RMSD bond length	0.005
RMSD bond angle	0.923
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.850
High resolution limit [Å]	2.790	7.590	2.800
Rmerge	0.234	0.103
Rmeas	0.255	0.123
Rpim	0.175	0.067	0.888
Number of reflections	36276	1853	1755
<I/σ(I)>	2.4
Completeness [%]	98.3	96.7	96.4
Redundancy	3.4	3.2	3.3
CC(1/2)		0.973	0.338

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.1 M Ammonium Acetate, 0.1 M Bis-Tris pH 5.5, 17% w/v PEG 10000