7CCX
Crystal structure of the holo form of human hydroxymethylbilane synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-08 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.411, 80.815, 109.194 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.150 - 1.840 |
| R-factor | 0.1961 |
| Rwork | 0.194 |
| R-free | 0.24720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ecr |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.745 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.150 | 1.950 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Number of reflections | 53490 | 15522 |
| <I/σ(I)> | 10.73 | 2.31 |
| Completeness [%] | 98.2 | 92 |
| Redundancy | 4 | 2.8 |
| CC(1/2) | 0.997 | 0.915 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 293 | PEG 3350, diammonium hydrogen citrate, dithiothreitol |
