7CBY
Structure of FOXG1 DNA binding domain bound to DBE2 DNA site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-17 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.126, 61.446, 73.594 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.871 - 1.646 |
R-factor | 0.1575 |
Rwork | 0.156 |
R-free | 0.18220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ako |
RMSD bond length | 0.009 |
RMSD bond angle | 1.117 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.900 | 1.700 |
High resolution limit [Å] | 1.640 | 1.640 |
Rmeas | 0.035 | |
Rpim | 0.312 | |
Number of reflections | 26897 | 2635 |
<I/σ(I)> | 20.7 | |
Completeness [%] | 98.9 | |
Redundancy | 13.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 80 mM NaCl, 40 mM Sodium cacodylate, pH 6.0, 45% 2-Methyl-2,4-pentanediol, 12 mM spermine |