7C6B
Crystal structure of Ago2 MID domain in complex with 6-(3-(2-carboxyethyl)phenyl)purine riboside monophosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 |
Unit cell lengths | 47.280, 66.460, 40.420 |
Unit cell angles | 107.06, 95.63, 86.40 |
Refinement procedure
Resolution | 38.510 - 1.700 |
R-factor | 0.1962 |
Rwork | 0.194 |
R-free | 0.22860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6L45 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.717 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.8.2) |
Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 38.510 | 38.510 | 1.800 |
High resolution limit [Å] | 1.700 | 5.100 | 1.700 |
Rmerge | 0.191 | 0.126 | 1.100 |
Rmeas | 0.215 | 0.143 | 1.307 |
Total number of observations | 228418 | ||
Number of reflections | 51572 | 1902 | 8073 |
<I/σ(I)> | 5.44 | 8.48 | 1.44 |
Completeness [%] | 99.8 | 99.1 | 99.5 |
Redundancy | 4.429 | 4.632 | 3.351 |
CC(1/2) | 0.982 | 0.986 | 0.601 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277.15 | 0.1M imidazole pH8.0, 0.2M NaCl, 0.46M NaH2PO4, 1.84M K2HPO4 |