7BYI

Structure of SHMT2 in complex with CBX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9778
Spacegroup name	P 65 2 2
Unit cell lengths	158.620, 158.620, 209.427
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.500 - 2.760
R-factor	0.2453
Rwork	0.242
R-free	0.31080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6h3c
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.840
High resolution limit [Å]	2.760	2.760
Number of reflections	38383	6297
<I/σ(I)>	18.25
Completeness [%]	100.0
Redundancy	21.2
CC(1/2)	0.973	0.884

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M Sodium dihydrogen phosphate pH 6.5, 16% PEG 2000,2.5mM Carbenoxolone Sodium