7BTD

Crystal structure of Rheb Y35N mutant bound to GppNHp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9785
Spacegroup name	C 2 2 21
Unit cell lengths	97.637, 102.962, 47.715
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.000
R-factor	0.168
Rwork	0.166
R-free	0.20830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xts
RMSD bond length	0.009
RMSD bond angle	1.217
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0123)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.010	50.000	2.070
High resolution limit [Å]	2.000	4.310	2.000
Rmerge	0.100	0.061	0.547
Rmeas	0.108	0.066	0.596
Rpim	0.040	0.025	0.232
Number of reflections	16650	1776	1622
<I/σ(I)>	4.6
Completeness [%]	99.8	99.7	99.1
Redundancy	7.1	7	6.3
CC(1/2)		0.996	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	290	0.2 M Ammonium acetate: 0.1 M HEPES, pH 7.5: 25% PEG 3350.