7BQU

Cereblon in complex with SALL4 and (S)-thalidomide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2019-05-26
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.000
Spacegroup name	C 2 2 21
Unit cell lengths	70.962, 92.718, 43.986
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.674 - 1.900
R-factor	0.1929
Rwork	0.191
R-free	0.23580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tz4 5yj0
RMSD bond length	0.011
RMSD bond angle	1.375
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER (Ver 1.15.1_3469)
Refinement software	PHENIX (Ver 1.15.1_3469)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.990	1.940
High resolution limit [Å]	1.900	1.900
Rmeas	0.158	1.310
Rpim	0.044	0.363
Number of reflections	11808	746
<I/σ(I)>	12	2.5
Completeness [%]	100.0	100
Redundancy	12.9	12.8
CC(1/2)	0.997	0.826

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	27% PEG 4000, 0.1 M sodium acetate (pH 5.5), 0.1 M magnesium chloride