7BPH
Crystal structure of GppNHp-bound GNAS in complex with the cyclic peptide inhibitor GN13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-12-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.999965 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.905, 78.332, 80.043 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.450 - 1.570 |
| R-factor | 0.1987 |
| Rwork | 0.197 |
| R-free | 0.22910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6au6 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.387 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.600 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.074 | 1.040 |
| Rmeas | 0.086 | 0.972 |
| Rpim | 0.023 | 0.405 |
| Number of reflections | 58703 | 57627 |
| <I/σ(I)> | 27.3 | 1.32 |
| Completeness [%] | 96.9 | |
| Redundancy | 12.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 0.1M HEPES 7.2, 20% PEG 4000, 10% v/v 2-propanol |
