7BK4
Crystal structure of RXRalpha ligand binding domain in complex with a fragment of the TIF2 coactivator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-19 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 179.132, 64.659, 48.514 |
| Unit cell angles | 90.00, 95.28, 90.00 |
Refinement procedure
| Resolution | 48.310 - 2.800 |
| R-factor | 0.2473 |
| Rwork | 0.246 |
| R-free | 0.26540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kwy |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.372 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (7.1.005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.310 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.069 | 0.187 |
| Number of reflections | 13251 | 1338 |
| <I/σ(I)> | 16.52 | |
| Completeness [%] | 96.1 | |
| Redundancy | 3.1 | |
| CC(1/2) | 0.995 | 0.678 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Na Hepes, 0.2 M sodium acetate pH 7.5, 27% PEG 3350 |
