7BI6
PI3KC2a core in complex with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.124, 133.371, 152.709 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.560 - 2.750 |
| R-factor | 0.2465 |
| Rwork | 0.244 |
| R-free | 0.28910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7bi2 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.147 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.560 | 2.910 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Number of reflections | 30416 | 4878 |
| <I/σ(I)> | 9.57 | |
| Completeness [%] | 98.8 | 97.9 |
| Redundancy | 4.1 | |
| CC(1/2) | 0.997 | 0.424 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | 0.1MTris, 8-6% PEG3350, 0.2-0.1M MgSO4 |
