7BFI
A double-histidine mutant of HSP47 slows down client release at low pH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-22 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.704, 77.406, 122.343 |
| Unit cell angles | 90.00, 96.60, 90.00 |
Refinement procedure
| Resolution | 78.180 - 2.440 |
| R-factor | 0.2182 |
| Rwork | 0.217 |
| R-free | 0.24820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zha |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.596 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.180 | 2.590 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmerge | 0.069 | 0.874 |
| Rmeas | 0.070 | 0.940 |
| Number of reflections | 52937 | 8413 |
| <I/σ(I)> | 11.77 | |
| Completeness [%] | 96.9 | 95.7 |
| Redundancy | 2.73 | 2.65 |
| CC(1/2) | 0.998 | 0.635 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 8 % Tacsimate, 18% PEG 3350, 0.1 M HEPES, pH 7.5 |
